Identification And Characterization Of Phosphate Transporters Genes In Sugarcane (saccharum Spp.).
(Almeida, Raul Santin)
Identification and characterization of phosphatetransporters genes in sugarcane (Saccharum spp.).Brazil is rsponsible for 25% of the world production of sugarcane(Saccharum spp.), and the economic importance of this crop is relatedto its increasing use as energy source and sugar. Traditional geneticbreeding programs are hamperd by the genomic complexity, derived fromits multi-specific origing adn polyploidy. The availability ofinorganic phosphorous (Pi) in agri-ecosystems is one of the largestlimiation for plant growth and yield, because Pi uptake by the rootsdepends on the variation of the concentration of labile phosphorous inthe soil and against an eletric and concentration gradient. Forintensive agriculture, inorganic and organic sources of phosphorous arelargely used, but part of this Pi is not recoverd from the soil,because around 80% becomes adsorbed or complexed to the organicfraction, together with heavy metals residues (ex: Zn, Cd, Ni or Cu).Genes of low affinity phosphate transporters are constitutivelyexpressed and work under higher concentrations of available Pi.Sequences of high affinity phosphate transporters already described(PHO84 of Saccharomyces cerevisiae; PHO-5 of Neurospora crassa; GvPT ofGlomus versiforme; AtPT1 and AtPT2 of Arabidopsis thaliana; LePT1 andLePT2 of Lycopersicum esculentum; StPT1 and StPT2 of Solanum tuberosum)encoded a Pi transporter protein with 12 conserve d transmembranedomains. Their expression in plants occur preferntially ion roots underphosphorous deficiency. Using the Sequences of these high affinityphosphate transporter genes, ESTs sequences with high identity wereidentified in the sugarcane genome project, especially the clusterSCEQRT1028B07.g, with a complete cDNA, which deducted sequence codedfor a protein with 541 amino acids, and 12 transmembrane domais,typical of these transporters.
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