Application Of Molecular Genetics For Diagnosis Of Neurocysticercosis And Genes Discovery In Taeni
(Almeida, Carolina Rodrigues de)
Application of molecular genetics for diagnosis of neurocysticercosis and genes discovery in Taenia soliumNeurocysticercosis is a frequent parasitic infection of the centralnervous system, mainly in developing countries. High costs of magneticresonance images (nowadays the most accurate method of diagnosis) maybe prohibitive in these regions and the interpretation of the multipleimmunologic tests available is often difficult. We demonstrate in thiswork that Taenia solium DNA is present in the cerebrospinal fluid (CSF)of patients and enabled the correct diagnosis of 29/30 patients (96.7%) with 100\% specificity through the PCR methodology. The presence ofparasite DNA in the CSF of patients may have important consequencesover the diagnosis of this important disease. Besides, we enlargesignificant the knowledge about Taenia´s transcriptome producing thefirst 2880 ESTs (Expressed Sequence Tags) of Taenia solium cysticerci.We were able to found at least seven new important genes that may be aimportant role in a battle against neurocysticercosis because throughthem it is possible identify considerable aspects of this disease, eventhough establishing other and efficient strategies of prevention,founding new antigens to improve the immunodiagnosis or building avaccine to protect the hosts that carry on this important parasite.
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